FUT3 antibody

Catalog No. ABIN7865636

This Mouse Monoclonal antibody specifically detects FUT3 in WB, ELISA, IHC, ICC, IF and FACS. It exhibits reactivity toward Human and Cow.

Quick Overview for FUT3 antibody (ABIN7865636)

Target: FUT3 (Fucosyltransferase 3 (Galactoside 3(4)-L-Fucosyltransferase, Lewis Blood Group) (FUT3))

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This FUT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: EBS-CD-049

Product Details anti-FUT3 Antibody

Purpose: anti-CD174 mouse monoclonal, EBS-CD-049, purified

Purification: affinity chromatography

Immunogen: neuraminidase-treated live T-47D breast carcinoma cells

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: assay dependent

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for FUT3

Target: FUT3 (Fucosyltransferase 3 (Galactoside 3(4)-L-Fucosyltransferase, Lewis Blood Group) (FUT3))

Alternative Name: CD174

Background: EBS-CD-049 recognizes LeY (CD174) and to some extent Leb. EBS-CD-049 displays no cross-reactivity with blood group antigens A, B, Lea, LeX, Sialyl-Lea, Sialyl-LeX, nor with H type 1, type 2, or type 3, nor with 74 other synthetic oligo- and mono-saccharides tested (minimal reactivity exists to Globo-H). In addition, a protein conformational epitope on human and bovine histone H1 is detected (mimicry). Histones H2A, H2B, H3 and H4 are not detected. Positive control: Breast or colorectal cancer.