GNRHR antibody (Extracellular Domain)

Catalog No. ABIN7865640

The Mouse Monoclonal anti-GNRHR antibody is suitable to detect GNRHR in samples from Human and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for GNRHR antibody (Extracellular Domain) (ABIN7865640)

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GNRHR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: F1G4

Product Details anti-GNRHR Antibody

Binding Specificity: AA 1-29, Extracellular Domain

Purpose: anti-GnRH-receptor mouse monoclonal, F1G4, purified

Purification: affinity chromatography

Immunogen: BSA-conjugated peptide corresponding to amino acids 1-29 (MANSASPEQNQHCSAINNSIPLMQGNLPY) of human GnRH receptor extracellular domain

Isotype: IgG1 kappa

Application Details

Application Notes:

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM Tris with 1 mM EDTA pH 9.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for GNRHR

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Alternative Name: GnRH-receptor

Background: F1G4 reacts with GnRH receptors in the anterior pituitary. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinising hormone (LH) as well as follicle-stimulating hormone (FSH). The receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular, and intracellular loops characteristic of G protein couple receptors. Some cancers like ovarian and breast cancers sometimes carry. Positive control: Human pituitary gland, ovarian and breast cancers.