hCG Holo antibody

Catalog No. ABIN7865643

The Mouse Monoclonal anti-hCG Holo antibody is suitable to detect hCG Holo in samples from Human. It has been validated for IHC.

Quick Overview for hCG Holo antibody (ABIN7865643)

Target: hCG Holo

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HCGh25

Product Details

Purpose: anti-hCG Holo mouse monoclonal, HCGh25, purified

Purification: affinity chromatography

Immunogen: purified hCG

Isotype: IgG1 kappa

Application Details

Application Notes:

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details

Target: hCG Holo

Background: HCGh25 is very special because it reacts ONLY with intact-hCG and not with either free alpha- or free beta-chain of hCG. hCG is a glycoprotein composed of two non-identical, non-covalently linked polypeptide chains. The alpha subunit is identical among hCG, TSH, FSH and LH. While hCG is secreted in large quantities by normal trophoblasts, it is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. Besides trophoblastic tumors e.g. choriocarcinoma, large cell carcinoma, adenocarcinoma and squamous cell carcinoma of the lung are also positive in 90 % , 60 % and 20 % of cases respectively. hCG expression by non-trophoblastic tumors may indicate aggressive behavior. Positive control: JAR or TT Cells. Placenta.