Histone H1 antibody

Catalog No. ABIN7865649

The Mouse Monoclonal anti-Histone H1 antibody is suitable to detect Histone H1 in samples from Human, Mouse and Rat. It has been validated for IF, IHC, FACS and ICC.

Quick Overview for Histone H1 antibody (ABIN7865649)

Target: Histone H1

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: AE-4

Product Details anti-Histone H1 Antibody

Purpose: anti-Histone H1 mouse monoclonal, AE-4, purified

Purification: affinity chromatography

Immunogen: human leukemia biopsy cells

Isotype: IgG2a kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for Histone H1

Target: Histone H1

Background: AE-4 recognizes histone H1 in the nuclei of animal and plant cells. It produces a speckled nuclear staining pattern in normal and malignant cells. Positive control: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma or tonsil.