ITGAL antibody

Catalog No. ABIN7865659

The Mouse Monoclonal anti-ITGAL antibody (Clone 87-6F9) (ABIN7865659) specifically detects ITGAL in FACS, WB, IHC, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for ITGAL antibody (ABIN7865659)

Target: ITGAL (Integrin alpha-L (ITGAL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGAL antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 87-6F9

Product Details anti-ITGAL Antibody

Purpose: anti-CD11a mouse monoclonal, 87-6F9, purified

Purification: affinity chromatography

Immunogen: stimulated human leucocytes

Isotype: IgG2b kappa

Application Details

Application Notes:

ICC/IF_dilution: assay dependent

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: CD11a

Background: 87-6F9 Reacts with CD11a, a transmembrane molecule with 1145 amino acid residues and a MW of 180 kDa. CD11a is expressed on lymphocytes, granulocytes, monocytes and macrophages. Levels on memory T-cells tend to increase. CD11a plays a key role in mediating leukocyte adhesion to endothelium during inflammatory response through binding to ICAM 1 (CD54). Other ligands are ICAM-2 and ICAM-3. It is also involved in many other T-cell functions and immune phenomena. When paired with CD18, it forms the integrin alphaLbeta2 adhesion. 87-6F9 potently blocks LFA-1 dependent homotypic cell aggregation and was typed in the IVth International Leucocyte Typing Workshop. Positive control: Human leucocytes.

Pathways: Activated T Cell Proliferation, Integrin Complex