ASRGL1 antibody

Catalog No. ABIN7865675

This Mouse Monoclonal antibody specifically detects ASRGL1 in IHC, WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for ASRGL1 antibody (ABIN7865675)

Target: ASRGL1 (Isoaspartyl Peptidase/L-Asparaginase (ASRGL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ASRGL1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Clone: 7B6

Product Details anti-ASRGL1 Antibody

Purpose: anti-CRASH mouse monoclonal, 7B6, purified

Purification: affinity chromatography

Immunogen: with CRASH recombinant protein and synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes:

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for ASRGL1

Target: ASRGL1 (Isoaspartyl Peptidase/L-Asparaginase (ASRGL1))

Alternative Name: CRASH

Background: 7B6 reacts with CRASH, a 308 AA glycoprotein sharing 32 % homology with human asparaginase and identical to Asparaginase-Like Protein (ALP) found in rat sperm. CRASH is found in a variety of tumors but only occasionally in normal tissues. Especially ovarian cancers are highly positive. CRASH is further found in brain tumors, some colonic cancers, some lung cancers, some prostate cancers, uterine cancers, some breast cancers and some thyroid cancers. It was not found in serum. In Western blot 3 bands are demonstrated of 45 kDa, 28 kDa and 15 kDa. Positive control: Ovarian cancer, KM22 breast cancer cells.