KRT4 antibody

Catalog No. ABIN7865679

The Mouse Monoclonal anti-KRT4 antibody is suitable to detect KRT4 in samples from Human, Rat, Mouse, Dog, Cat, Rabbit, Goat, Guinea Pig, Monkey, Chicken, Ferret and Xenopus laevis. It has been validated for WB, IHC, IF, FACS and ICC.

Quick Overview for KRT4 antibody (ABIN7865679)

Target: KRT4 (Keratin 4 (KRT4))

Reactivity: Human, Rat, Mouse, Dog, Cat, Rabbit, Goat, Guinea Pig, Monkey, Chicken, Ferret, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: C-11

Product Details anti-KRT4 Antibody

Purpose: anti-Keratin 4,5,6,8,10,13,18 mouse monoclonal, C-11, purified

Purification: affinity chromatography

Immunogen: cytoskeleton preparation of human A431 carcinoma cell line

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 or digestion with trypsin at 1 mg/mL PBS recommended)

WB_dilution: 1:100 (1 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for KRT4

Target: KRT4 (Keratin 4 (KRT4))

Alternative Name: Keratin 4

Background: C11 reacts with keratins : 4,5,6,8,10,13 and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Positive control: A431 cells, skin, lung.