Keratin 5/14 antibody

Catalog No. ABIN7865680

The Mouse Monoclonal anti-Keratin 5/14 antibody (Clone EBS-IF-001) (ABIN7865680) specifically detects Keratin 5/14 in IHC, ICC, IF, FACS and WB. The antibody is reactive with Human and Cat samples.

Quick Overview for Keratin 5/14 antibody (ABIN7865680)

Target: Keratin 5/14 (KRT5/14)

Reactivity: Human, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 5/14 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB)

Clone: EBS-IF-001

Product Details anti-Keratin 5/14 Antibody

Purpose: anti-Keratin 5+14 mouse monoclonal, EBS-IF-001, purified

Purification: affinity chromatography

Immunogen: cytokeratin-enriched extract of feline tongue epithelium

Isotype: IgG2a kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C

WB_dilution: assay dependent

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for Keratin 5/14

Target: Keratin 5/14 (KRT5/14)

Alternative Name: Keratin 5+14

Background: EBS-IF-001 Does not react with keratin polypeptides in immunoblotting. The specificity for keratin was established on the basis of antibody reactivity with intracytoplasmic intermediate filaments in epithelial, vimentin negative, human and feline cells. Distribution of the epitope strongly suggests that EBS-IF-001 reacts with keratins 5 and 14, most probably with a heterotypic complex formed by these keratins. EBS-IF-001 reacts positively with basal cells of all stratified epithelia, with myoepithelial cells and with most squamous cell carcinomas. It is useful in immunohistochemistry for typing of basal cells. Positive control: Tongue.