Laminin gamma 1 antibody

Catalog No. ABIN7865685

The Rat Monoclonal anti-Laminin gamma 1 antibody is suitable to detect Laminin gamma 1 in samples from Human, Mouse, Rat, Monkey and Pig. It has been validated for WB, IHC, IF, ICC and FACS.

Quick Overview for Laminin gamma 1 antibody (ABIN7865685)

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Reactivity: Human, Mouse, Rat, Monkey, Pig

Host: Rat

Clonality: Monoclonal

Conjugate: This Laminin gamma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: A5

Product Details anti-Laminin gamma 1 Antibody

Purpose: anti-Laminin γ1 rat monoclonal, A5, purified

Purification: affinity chromatography

Immunogen: laminin preparation from murine EHS cells

Isotype: IgG2a kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for Laminin gamma 1

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Alternative Name: Laminin gamma1

Background: Laminins are large hetero-trimeric, non-collagenous glycoproteins found in basement membranes and composed of α, β, and γ chains. A5 reacts specifically with γ chain 1. Alterations of basement membrane integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. Positive control: Normal colon or carcinomas.