NUMA1 antibody

Catalog No. ABIN7865708

This Mouse Monoclonal antibody specifically detects NUMA1 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for NUMA1 antibody (ABIN7865708)

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NUMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: EBS-C-002

Product Details anti-NUMA1 Antibody

Purpose: anti-NuMA mouse monoclonal, EBS-C-002, purified

Purification: affinity chromatography

Immunogen: live Ls 174T cells (colon carcinoma)

Isotype: IgM kappa

Application Details

Application Notes:

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM Tris with 1 mM EDTA pH 9.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Alternative Name: NuMA

Background: EBS-C-002 reacts with NuMA or Nuclear Mitotic Apparatus protein, which at the onset of mitosis redistributes from the nucleus to two centrosomal structures at the poles of the mitotic spindle, where it plays a vital role in establishing and maintaining its bipolar structure. After anaphase the protein redistributes from the spindle polar region into the reforming nucleus and concentrates initially at the site where nuclear lamins and perichomatin have been reported to assemble. In contrast to mitotic cells, post-mitotic neurons display NuMA both in the nucleus and in the cytoplasm. Due to release from dead cells, NuMA is also used as oncological marker in serum and urine. In addition, chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia. Positive control: Human toncil.

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase