Nucleolar Antigen antibody
Quick Overview for Nucleolar Antigen antibody (ABIN7865709)
Target
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- anti-Nucleolar antigen mouse monoclonal, AE-3, purified
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Purification
- affinity chromatography
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Immunogen
- nuclei of myeloid leukemia biopsy cells
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Isotype
- IgG1 kappa
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Application Notes
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ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 100 μg/mL
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Buffer
- PBS with 0.02 % sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Storage Comment
- store at 2 - 8 °C
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- Nucleolar Antigen
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Background
- AE-3 recognizes a nucleolar antigen expressed in nucleoli of human cells. AE-3 can also be used as a marker of the nucleolus in subcellular fractions. Positive Control: Human tonsil.
Target
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