PLAP antibody

Catalog No. ABIN7865713

The Mouse Monoclonal anti-PLAP antibody (Clone H7E8) (ABIN7865713) specifically detects PLAP in WB, IHC, ELISA, FACS and IP. The antibody is reactive with Human samples.

Quick Overview for PLAP antibody (ABIN7865713)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: H7E8

Product Details anti-PLAP Antibody

Purpose: anti-Placental alkaline phosphatase (PLAP) mouse monoclonal, H7E8, purified

Purification: affinity chromatography

Immunogen: PLAP, purified from FS phenotype human placenta

Isotype: IgG2a kappa

Application Details

Application Notes:

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Alternative Name: Placental alkaline phosphatase (PLAP)

Background: PLAP is a tissue specific, throphoblast-derived, 58 kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallated glycoprotein, only found in humans, orangutans and chimpanzees, that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific APs in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98 % homologous. Non tissue specific AP is found in kidney, liver and bone. H7E8 binds equally well to all common allelic variants (S,F, FS and I) of PLAP as to AP from normal human testis, while antibody F5C2 reacts with some samples of normal human testis only. H7E8 can be used as epitope-defined marker in germ cell tumors and is applicable on frozen sections directly as well as on paraffin sections after a heat induced antigen retrieval step. H7E8 can be used both as coating as well as tracer antibody in the same ELISA to detect PLAP in serum of S, F, FS and I phenotypes. H7E8 can also be used for immunosorbent purification and for radioimmuno-localization. Positive control: human placenta.