Retinol-Binding Protein antibody

Catalog No. ABIN7865727

The Mouse Monoclonal anti-Retinol-Binding Protein antibody is suitable to detect Retinol-Binding Protein in samples from Mouse, Goat, Monkey, Rat and Rabbit. It has been validated for IHC, WB and ELISA.

Quick Overview for Retinol-Binding Protein antibody (ABIN7865727)

Target: Retinol-Binding Protein (RBP)

Reactivity: Mouse, Goat, Monkey, Rat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinol-Binding Protein antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Clone: G4E4

Product Details anti-Retinol-Binding Protein Antibody

Purpose: anti-Retinol Binding Protein mouse monoclonal, G4E4, purified

Purification: affinity chromatography

Immunogen: with human retinol binding protein purified from plasma

Isotype: IgG1 kappa

Application Details

Application Notes:

IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:100-1:200 (0.5-1 μg/mL)

IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for Retinol-Binding Protein

Target: Retinol-Binding Protein (RBP)

Alternative Name: Retinol Binding Protein

Background: G4E4 recognizes an epitope within the 74-182 C-terminal sequence (11kD peptide fragment) of human serum Cellular Retinol Binding Protein 1 (CRBP 1), a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. CRBP 1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver. Positive control: Liver.