ST6GAL1 antibody

Catalog No. ABIN7865739

The Mouse Monoclonal anti-ST6GAL1 antibody is suitable to detect ST6GAL1 in samples from Human. It has been validated for IHC, FACS, IF and ICC.

Quick Overview for ST6GAL1 antibody (ABIN7865739)

Target: ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ST6GAL1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: LN-1

Product Details anti-ST6GAL1 Antibody

Purpose: anti-CDw75 mouse monoclonal, LN-1, purified

Purification: affinity chromatography

Immunogen: nuclei from pokeweed mitrogen-stimulated PBL

Isotype: IgM kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for ST6GAL1

Target: ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))

Alternative Name: CDw75

Background: LN-1 reacts with CDw75, a neuraminidase sensitive cell surface sialoglycan which is present on the cell membrane and cytoplasm of germininal center B-cells and derived lymphomas. CDw75 has a function in cell adhesion and is the ligand for CD22. LN-1 reacts with RBC precursors in bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with gioblastomas and astrocytoma’s, and also Reed Sternberg cells in Hodgkin’s disease. Positive control: HeLa or Daudi cells. Germinal center B-cell in a lymph node or tonsil. Use LN-1’s ability to stain the surface of erythrocytes as an internal positive control.