Thyroglobulin antibody

Catalog No. ABIN7865744

This Mouse Monoclonal antibody specifically detects Thyroglobulin in IHC, WB, ELISA, FACS, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for Thyroglobulin antibody (ABIN7865744)

Target: Thyroglobulin (TG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thyroglobulin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: EBS-O-170

Product Details anti-Thyroglobulin Antibody

Purpose: anti-Thyroglobulin mouse monoclonal, EBS-O-170, purified

Purification: affinity chromatography

Immunogen: human thyroglobulin

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:100-1:200 (0.5-1 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for Thyroglobulin

Target: Thyroglobulin (TG)

Alternative Name: Thyroglobulin

Background: EBS-O-170 reacts with thyroglobulin, a 660 kDa dimeric pre-protein with multiple glycosylation sites. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. The vast majority of follicular carcinomas of the thyroid are positive for thyroglobulin even though sometimes only focally. Poorly differentiated thyroid carcinomas are frequently thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. EBS-O-170 has a high affinity for thyroglobulin (Ka = 8.6 x 10^-9). Positive control: Human thyroid.

Pathways: Thyroid Hormone Synthesis