CD3 antibody

Catalog No. ABIN7866123

The Mouse Monoclonal anti-CD3 antibody (Clone M2AB) (ABIN7866123) specifically detects CD3 in FACS. The antibody is reactive with Human samples.

Quick Overview for CD3 antibody (ABIN7866123)

Target: CD3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: M2AB

Product Details anti-CD3 Antibody

Purpose: Mouse anti Human CD3

Characteristics: The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is present on 65-85 % of thymocytes and has a mitogenic effect on peripheral blood T cells. Identification of human T cells expressing the 22-28,000 M.W. surface antigen.

Purification: Protein A/G Chromatography

Immunogen: CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes.

Isotype: IgG1

Application Details

Application Notes: PBMC: Add10 μL of MAB/106 PBMC in 100 μL PBS. Mix gently and incubate for 15 minutes at 2o to 8o C. Wash twice with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 μL of MAB/100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Use as above, however use only 5 μL per test rather than 10 μL. Excites at 488nm and emits at 670nm. Store protected from light.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD1E antigen

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway