Feimin antibody

Catalog No. ABIN7866182

The Mouse Monoclonal anti-Feimin antibody is suitable to detect Feimin in samples from Mouse and Human. It has been validated for ELISA, IP, WB and SDS.

Quick Overview for Feimin antibody (ABIN7866182)

Target: Feimin

Reactivity: Mouse, Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB), SDS-PAGE (SDS)

Product Details

Purpose: Anti-Feimin (MOUSE) Monoclonal Antibody

Cross-Reactivity (Details): A BLAST analysis of the sequence of the immunogen shows 100% identity with mouse, 97% bovine, and 94% human. This product reacts with mouse, and human Feimin isoform 1 and 2. Reactivity against homologues from other sources is not known.

Purification: Anti-Feimin Monoclonal Antibody is Protein A purified

Sterility: Sterile filtered

Immunogen: This monoclonal antibody was prepared in mice after repeated immunizations with recombinant mouse Feimin protein.

Isotype: IgG2b, kappa

Application Details

Application Notes: Anti-Feimin (MOUSE) Antibody has been tested by ELISA, Western Blot, and Immunoprecipitation. The antibody detects both human and mouse feimin protein from overexpressing cell lysates (using HEK293T). A band approximately 17kDa for isoform 2 and 20kDa fo

Comment:

ELISA Dilution: 1:265,000-1:409,000
Western Blot Dilution: 41:5000br />Immunoprecipitation Dilution: 40 ng/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.16 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Antibody is stable for

Expiry Date: 12 months

Target Details

Target: Feimin

Background: Background: The Feimin protein, also known as UPF0461 protein or C5orf24, involves regulatory roles within the cell and its exact role is under investigation. Feimin is a newly discovered myokine that complements insulin by fine-tuning glucose levels and boosting exercise capacity, especially under conditions where insulin is less effective. Highly conserved Feimin functions in both glucose homeostasis and has a direct role in muscle thermogenesis. Feimin can be secreted by muscle and act as a myokine where it can affect tissues by engaging MERTK receptor thereby activating AKT. Cellular Feimin (cFeimin) functions as a transcription regulator by interacting with FOXC2 protein, where it helps to enhance exercise performance by reducing thermogenesis.
Synonyms: Chromosome 5 Open Reading Frame 24, UPF0461 Protein C5orf24, FLJ37562, Feimin

Gene ID: 134553

NCBI Accession: NP_001129058.1

UniProt: Q80X32