BrdU antibody (Biotin)

Catalog No. ABIN7867111

The Biotin-conjugated Mouse Monoclonal anti-BrdU antibody (Clone Bu20a) (ABIN7867111) specifically detects BrdU in FACS.

Quick Overview for BrdU antibody (Biotin) (ABIN7867111)

Target: BrdU (Bromodeoxyuridine (BrdU))

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: Bu20a

Product Details anti-BrdU Antibody (Biotin)

Purpose: Anti-5-bromodeoxyuridine (BrdU)-Biotin Conjugated Monoclonal Antibody (Clone:Bu20a)

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen: Bromodeoxyuridine conjugated to BSA

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL, Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: 5-bromodeoxyuridine (BrdU)

Target Type: Chemical

Background:

Synonyms: 5-bromodeoxyuridine, Bu20a

Target Background: Bromodeoxyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Specificity: The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. It cross-reacts with EdU.

Gene ID: 35