NEFH antibody

Catalog No. ABIN7867384

The Mouse Monoclonal anti-NEFH antibody (Clone 2F11 ) (ABIN7867384) specifically detects NEFH in WB and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for NEFH antibody (ABIN7867384)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 2F11 

Product Details anti-NEFH Antibody

Purpose: Neurofilament (H+L) (Neuronal Marker) Antibody (Clone: 2F11)

Immunogen: Human NF-H from isolated brain cells

Isotype: IgG1, kappa

Application Details

Application Notes: Immunohistochemistry (IHC) : Tested Dilution: 1-2 μg/mL 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes. Western Blot (WB) 2-4 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Nonhazardous. No MSDS required.

Expiry Date: 24 months

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: NEFH

Background:

Synonyms: NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200 kDa, Neurofilament Triplet H Protein, NF-H, NF200. 68 kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68 kDa, Neurofilament light polype

Target Background:   This MAb reacts with a 200 kDa and 68 kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Gene ID: 4744

UniProt: P12036