IFT20 antibody (AA 1-132)

Catalog No. ABIN7868098

The Rabbit Polyclonal anti-IFT20 antibody (ABIN7868098) specifically detects IFT20 in WB, ELISA, IHC, IF and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for IFT20 antibody (AA 1-132) (ABIN7868098)

Target: IFT20 (Intraflagellar Transport 20 Homolog (IFT20))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFT20 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-IFT20 Antibody

Binding Specificity: AA 1-132

Purpose: IFT20 Antibody / Intraflagellar transport protein 20 homolog

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human IFT20 recombinant protein (Position: M1-K132) was used as the immunogen for the IFT20 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the IFT20 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the IFT20 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for IFT20

Target: IFT20 (Intraflagellar Transport 20 Homolog (IFT20))

Alternative Name: IFT20

Background: IFT20 antibody detects Intraflagellar transport protein 20 homolog, a component of the intraflagellar transport (IFT) complex required for cilium assembly, maintenance, and cargo trafficking. The UniProt recommended name is Intraflagellar transport protein 20 homolog (IFT20). This small protein serves as a crucial adaptor that links vesicular trafficking machinery to the IFT complex, ensuring proper delivery of membrane and signaling components to the cilium.

Functionally, IFT20 antibody identifies a 132-amino-acid protein localized to the Golgi apparatus and the base of cilia. IFT20 associates with the IFT-B complex and interacts with proteins such as IFT57, IFT88, and GMAP210 to coordinate transport of ciliary cargo from the Golgi to the basal body. It plays essential roles in ciliogenesis, ciliary membrane protein sorting, and Hedgehog signaling. IFT20 functions both in ciliated cells, such as renal epithelial cells and photoreceptors, and in non-ciliated cells, where it contributes to vesicle trafficking and immune synapse formation.

The IFT20 gene is located on chromosome 17q25.3 and is ubiquitously expressed in tissues with active ciliary assembly, including kidney, lung, retina, and brain. Expression is dynamically regulated during cell cycle progression and development, reflecting its importance in signaling and organelle biogenesis.

Pathologically, mutations or loss of IFT20 disrupt cilium formation and function, leading to a spectrum of ciliopathies such as polycystic kidney disease, retinal degeneration, and developmental disorders. Altered expression may also affect immune function, as IFT20 participates in T-cell receptor recycling. Research using IFT20 antibody supports studies in cilia biology, vesicular trafficking, and developmental signaling pathways.

IFT20 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect ciliary transport proteins. NSJ Bioreagents provides IFT20 antibody reagents optimized for studies in ciliogenesis, Golgi-cilium communication, and membrane trafficking.

Structurally, Intraflagellar transport protein 20 homolog is a small coiled-coil protein that integrates into the IFT-B complex. Its N-terminal region anchors it to the Golgi, while the C-terminal region mediates interactions with IFT components. This structural configuration enables IFT20 to couple vesicular transport and ciliary assembly. This antibody aids in investigating IFT20's role in cellular signaling, ciliogenesis, and trafficking fidelity.

UniProt: Q8IY31

Pathways: Hedgehog Signaling