RAD51 antibody (AA 1-134)

Catalog No. ABIN7868113

The Mouse Monoclonal anti-RAD51 antibody (Clone RALPA-1) (ABIN7868113) specifically detects RAD51 in WB, FACS and IHC (p). The antibody is reactive with Human samples.

Quick Overview for RAD51 antibody (AA 1-134) (ABIN7868113)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAD51 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RALPA-1

Product Details anti-RAD51 Antibody

Binding Specificity: AA 1-134

Purpose: RAD51 Antibody

Purification: Protein G affinity chromatography

Immunogen: A portion of amino acids 1-134 from the human protein was used as the immunogen for this RAD51 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the RAD51 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the RAD51 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Alternative Name: RAD51

Background: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [RefSeq]

UniProt: Q06609

Pathways: DNA Damage Repair