OCT4 antibody (AA 1-134)

Catalog No. ABIN7868119

The Mouse Monoclonal anti-OCT4 antibody (Clone Oct4-3508) (ABIN7868119) specifically detects OCT4 in IHC (p). The antibody is reactive with Human samples.

Quick Overview for OCT4 antibody (AA 1-134) (ABIN7868119)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: Oct4-3508

Product Details anti-OCT4 Antibody

Binding Specificity: AA 1-134

Purpose: Oct4 Antibody / POU5F1

Purification: Protein A/G affinity

Immunogen: A portion of amino acids 1-134 was used as the immunogen for the Oct4. antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the Oct4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot the Oct4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: Oct4

Background: Oct4 Transcription factor that binds to the octamer motif (5). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

UniProt: Q01860

Pathways: Stem Cell Maintenance