Aconitase 1 antibody (AA 1-137)

Catalog No. ABIN7868131

The Rabbit Polyclonal anti-Aconitase 1 antibody (ABIN7868131) specifically detects Aconitase 1 in WB, ELISA, IHC (p), IF and FACS. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for Aconitase 1 antibody (AA 1-137) (ABIN7868131)

Target: Aconitase 1 (ACO1)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aconitase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Aconitase 1 Antibody

Binding Specificity: AA 1-137

Purpose: ACO1 Antibody / Aconitase 1

Purification: Affinity purified

Immunogen: Recombinant human protein (amino acids M1-D137) was used as the immunogen for the ACO1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ACO1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ACO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Aconitase 1

Target: Aconitase 1 (ACO1)

Alternative Name: ACO1

Background: The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants.

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis