PTRHD1 antibody (AA 1-140)

Catalog No. ABIN7868138

This Rabbit Polyclonal antibody specifically detects PTRHD1 in WB, ELISA, ICC, FACS, IF and IHC. It exhibits reactivity toward Mouse, Rat and Human.

Quick Overview for PTRHD1 antibody (AA 1-140) (ABIN7868138)

Target: PTRHD1 (Peptidyl-tRNA Hydrolase Domain Containing 1 (PTRHD1))

Reactivity: Mouse, Rat, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTRHD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-PTRHD1 Antibody

Binding Specificity: AA 1-140

Purpose: PTRHD1 Antibody / Peptidyl-tRNA hydrolase domain-containing protein 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human C2orf79/PTRHD1 recombinant protein (Position: M1-K140) was used as the immunogen for the PTRHD1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PTRHD1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PTRHD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PTRHD1

Target: PTRHD1 (Peptidyl-tRNA Hydrolase Domain Containing 1 (PTRHD1))

Alternative Name: PTRHD1

Background: PTRHD1 antibody detects Peptidyl-tRNA hydrolase domain-containing protein 1, a cytoplasmic enzyme-like protein implicated in tRNA metabolism, proteostasis, and neurological function. The UniProt recommended name is Peptidyl-tRNA hydrolase domain-containing protein 1 (PTRHD1). Although it contains a conserved hydrolase-like domain, PTRHD1 may function primarily as a regulator or adaptor rather than a catalytic enzyme, linking translation quality control with neuronal integrity.

Functionally, PTRHD1 antibody identifies a 220-amino-acid protein that associates with translational complexes and ubiquitin-proteasome pathways. PTRHD1 is proposed to participate in the recycling of prematurely released peptidyl-tRNAs during protein synthesis, preventing accumulation of stalled translation intermediates. It may also regulate proteasomal activity by binding to ubiquitin-like proteins and adaptors such as UBQLN4, thereby influencing protein degradation and quality control under cellular stress conditions.

The PTRHD1 gene is located on chromosome 2p23.3 and is expressed predominantly in brain, testis, and liver. Its expression pattern and domain composition suggest a dual role in neuronal homeostasis and proteostatic regulation. PTRHD1 may serve as a molecular bridge connecting translation fidelity to protein turnover and synaptic function.

Pathologically, biallelic loss-of-function mutations in PTRHD1 cause autosomal recessive early-onset parkinsonism with intellectual disability, highlighting its importance in maintaining neuronal health. Impaired PTRHD1 function results in accumulation of defective translation products and increased cellular stress, leading to neurodegeneration. Research using PTRHD1 antibody supports studies in translational control, proteostasis, and neurogenetic disorders.

PTRHD1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect small cytoplasmic regulatory proteins. NSJ Bioreagents provides PTRHD1 antibody reagents optimized for research in protein quality control, neuronal maintenance, and translation regulation.

Structurally, Peptidyl-tRNA hydrolase domain-containing protein 1 contains an N-terminal hydrolase-like domain similar to bacterial PTH enzymes but lacking key catalytic residues, suggesting a non-enzymatic regulatory role. The protein's C-terminal region supports interactions with ubiquitin-binding proteins. This antibody facilitates exploration of PTRHD1's role in neuronal proteostasis and translation fidelity.

UniProt: Q6GMV3