C6orf130 antibody (AA 1-152)

Catalog No. ABIN7868195

This Rabbit Polyclonal antibody specifically detects C6orf130 in WB, ELISA, IHC, ICC, FACS and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for C6orf130 antibody (AA 1-152) (ABIN7868195)

Target: C6orf130 (OARD1) (O-Acyl-ADP-Ribose Deacylase 1 (OARD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C6orf130 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-C6orf130 Antibody

Binding Specificity: AA 1-152

Purpose: OARD1 Antibody / O-acetyl-ADP-ribose deacetylase 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human C6orf130/OARD1 recombinant protein (Position: M1-L152) was used as the immunogen for the OARD1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the OARD1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the OARD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for C6orf130

Target: C6orf130 (OARD1) (O-Acyl-ADP-Ribose Deacylase 1 (OARD1))

Alternative Name: OARD1

Background: OARD1 antibody detects ADP-ribose glycohydrolase OARD1, encoded by the OARD1 gene on chromosome 6q14.2. OARD1 antibody is used in research on ADP-ribosylation, DNA repair, and chromatin biology. OARD1 is an enzyme that removes ADP-ribose from proteins, acting as a mono-ADP-ribosylhydrolase. This activity counteracts PARP-mediated ADP-ribosylation, a key post-translational modification involved in DNA repair, transcription, and stress signaling.

Structurally, OARD1 is a ~17 kDa protein containing a macrodomain, which mediates binding and hydrolysis of ADP-ribose groups. The macrodomain recognizes ADP-ribosylated substrates and catalyzes removal of the modification. OARD1 localizes to the nucleus, where it associates with chromatin and DNA repair complexes. Isoforms generated by alternative splicing provide additional regulatory functions.

Functionally, OARD1 regulates chromatin dynamics and DNA repair by reversing mono-ADP-ribosylation on histones and DNA repair proteins. This counter-regulation maintains genomic stability and prevents accumulation of aberrant ADP-ribose modifications. Knockdown of OARD1 leads to DNA damage sensitivity, chromatin defects, and altered transcription. Researchers use OARD1 antibody to study ADP-ribosylation signaling, chromatin regulation, and DNA repair pathways.

Clinically, OARD1 has been implicated in cancer and neurodegeneration. Altered ADP-ribosylation dynamics contribute to oncogenesis by disrupting DNA repair and transcriptional regulation. OARD1 may also influence response to PARP inhibitors, which are used to treat BRCA-deficient tumors. Mutations or dysregulation of OARD1 have been linked to neurological disorders involving DNA damage accumulation. NSJ Bioreagents provides OARD1 antibody to support cancer, neurology, and epigenetics research.

Experimentally, OARD1 antibody is used in western blotting to detect the ~17 kDa protein, in immunohistochemistry to study nuclear expression, and in chromatin immunoprecipitation to examine ADP-ribosylation regulation. Co-immunoprecipitation with OARD1 antibody identifies partners including PARP family members and DNA repair proteins.

UniProt: Q9Y530