C4orf49 antibody (AA 1-155)

Catalog No. ABIN7868216

This Rabbit Polyclonal antibody specifically detects C4orf49 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for C4orf49 antibody (AA 1-155) (ABIN7868216)

Target: C4orf49 (Chromosome 4 Open Reading Frame 49 (C4orf49))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C4orf49 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-C4orf49 Antibody

Binding Specificity: AA 1-155

Purpose: MGARP Antibody / Mitochondria-localized glutamic acid-rich protein

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human C4orf49/MGARP recombinant protein (Position: M1-E155) was used as the immunogen for the MGARP antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MGARP antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MGARP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for C4orf49

Target: C4orf49 (Chromosome 4 Open Reading Frame 49 (C4orf49))

Alternative Name: MGARP

Background: MGARP antibody detects Mitochondria-localized glutamic acid-rich protein, a mitochondrial membrane-associated protein that regulates organelle dynamics, energy metabolism, and neuronal development. MGARP functions as an adaptor involved in mitochondrial transport, positioning, and biogenesis, particularly in highly active tissues such as brain and retina. The MGARP antibody is widely used in studies of mitochondrial biology, neurodevelopment, and metabolic regulation, offering insight into mechanisms that control cellular energy distribution and organelle communication.

MGARP is encoded by the MGARP gene located on human chromosome 4q28.1. The protein is approximately 25 kilodaltons in size and characterized by an N-terminal mitochondrial targeting sequence and two C-terminal transmembrane domains that anchor it to the outer mitochondrial membrane. It interacts with proteins involved in mitochondrial motility and positioning, including Miro, TRAK1, and kinesin motor complexes. Through these interactions, MGARP mediates mitochondrial trafficking along microtubules and maintains energy homeostasis in neurons and photoreceptors.

The MGARP antibody is typically used to identify mitochondrial localization via immunofluorescence microscopy, where it produces a distinct punctate pattern consistent with the mitochondrial network. Western blot analysis detects a band near 38 kilodaltons. MGARP expression is enriched in neural tissues, endocrine organs, and energy-demanding cell types. Experimental evidence shows that it modulates mitochondrial fission and fusion dynamics, contributing to the distribution of mitochondria within axons and synaptic terminals. Loss or dysregulation of MGARP disrupts mitochondrial motility and impairs neuronal differentiation, emphasizing its importance in developmental neurobiology.

Beyond neuronal systems, MGARP has been implicated in steroid hormone biosynthesis and cellular stress adaptation. In steroidogenic cells, MGARP expression is upregulated by cAMP signaling and may influence mitochondrial cholesterol import. Its expression is responsive to hypoxia, oxidative stress, and glucose deprivation, indicating a role in cellular adaptation to metabolic stress. NSJ Bioreagents offers a validated MGARP antibody optimized for western blot, immunofluorescence, and immunohistochemistry, supporting research into mitochondrial dynamics, intracellular transport, and neuroendocrine function.

UniProt: Q8TDB4