GLOD5 antibody (AA 1-160)

Catalog No. ABIN7868224

The Rabbit Polyclonal anti-GLOD5 antibody (ABIN7868224) specifically detects GLOD5 in ELISA, FACS, IHC and WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GLOD5 antibody (AA 1-160) (ABIN7868224)

Target: GLOD5 (Glyoxalase Domain Containing 5 (GLOD5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLOD5 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-GLOD5 Antibody

Binding Specificity: AA 1-160

Purpose: GLOD5 Antibody / Glyoxalase domain-containing protein 5

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human GLOD5 recombinant protein (Position: M1-S160) was used as the immunogen for the GLOD5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GLOD5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GLOD5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GLOD5

Target: GLOD5 (Glyoxalase Domain Containing 5 (GLOD5))

Alternative Name: GLOD5

Background: GLOD5 antibody detects Glyoxalase domain-containing protein 5, an oxidoreductase-like protein potentially involved in detoxification of reactive carbonyl compounds. The UniProt recommended name is Glyoxalase domain-containing protein 5 (GLOD5). Although its enzymatic activity remains incompletely characterized, sequence analysis indicates homology to the glyoxalase and dioxygenase enzyme families responsible for cellular stress defense.

Functionally, GLOD5 antibody recognizes a cytoplasmic protein of approximately 160 amino acids that contains a metalloenzyme fold typical of glyoxalase domains. It is thought to play a role in the metabolism of reactive aldehydes and other cytotoxic intermediates generated during oxidative stress. GLOD5 expression may also contribute to maintaining redox balance and protecting cells from metabolic byproducts.

The GLOD5 gene is located on chromosome 9p13.2 and exhibits widespread expression in human tissues, including brain, kidney, and liver. Its evolutionary conservation among mammals suggests a physiological role in small-molecule metabolism or detoxification pathways. GLOD5 expression has been reported to increase under oxidative stress conditions, further linking it to cellular protection mechanisms.

Pathologically, altered GLOD5 expression may influence cell viability and stress adaptation. Dysregulation of glyoxalase-related enzymes is associated with diabetes, neurodegeneration, and cancer, though the specific contribution of GLOD5 remains under investigation. Using GLOD5 antibody enables detection of this lesser-known member of the glyoxalase family and aids exploration of its potential metabolic roles.

GLOD5 antibody is suitable for western blotting, immunocytochemistry, and immunohistochemistry to detect GLOD5 in cell and tissue extracts. NSJ Bioreagents offers GLOD5 antibody reagents validated for use in redox biology, metabolism, and cellular stress response research.

Structurally, GLOD5 contains a glyoxalase-like catalytic fold capable of coordinating divalent metal ions, although its active-site residues are not fully conserved. This antibody assists researchers in clarifying GLOD5's molecular function and contribution to oxidative metabolism.

UniProt: A6NK44