SPRY3 antibody (AA 1-162)

Catalog No. ABIN7868226

The Rabbit Polyclonal anti-SPRY3 antibody is suitable to detect SPRY3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for SPRY3 antibody (AA 1-162) (ABIN7868226)

Target: SPRY3 (Sprouty Homolog 3 (SPRY3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRY3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SPRY3 Antibody

Binding Specificity: AA 1-162

Purpose: SPRY3 Antibody / Sprouty RTK signaling antagonist 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SPRY3 recombinant protein (Position: M1-R162) was used as the immunogen for the SPRY3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SPRY3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SPRY3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SPRY3

Target: SPRY3 (Sprouty Homolog 3 (SPRY3))

Alternative Name: SPRY3

Background: SPRY3 antibody detects Sprouty RTK signaling antagonist 3, a negative regulator of receptor tyrosine kinase (RTK) signaling encoded by the SPRY3 gene located on the X and Y chromosomes (Xp22.33/Yp11.3). SPRY3 belongs to the sprouty family of cytoplasmic signaling modulators that fine-tune growth factor receptor signaling, particularly pathways initiated by fibroblast growth factors (FGFs), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF). It is expressed in brain, lung, placenta, and testes, where it regulates cell growth, differentiation, and angiogenesis.

Structurally, SPRY3 contains a conserved cysteine-rich C-terminal domain responsible for membrane association and a variable N-terminal region that mediates interaction with adapter proteins such as GRB2 and c-CBL. It belongs to the sprouty protein family (SPRY1-4), known for modulating Ras/MAPK signaling by interfering with RAF activation. Co-localization studies show SPRY3 associated with endosomal membranes and perinuclear regions, reflecting its regulatory role at signal transduction interfaces.

Functionally, SPRY3 acts as an inhibitor of the Ras/MAPK pathway, suppressing excessive signaling following receptor activation. It prevents cell overproliferation and supports proper developmental patterning. In neurons, SPRY3 regulates axonal branching and synaptic plasticity, while in endothelial cells it modulates angiogenic responses to VEGF. SPRY3 also interacts with E3 ubiquitin ligases such as c-CBL, influencing receptor internalization and degradation. Known downstream pathways include ERK1/2, PI3K-AKT, and Rho family GTPase signaling.

Expression of SPRY3 is developmentally regulated, with high levels observed in fetal brain and during vascular remodeling. Dysregulation or loss of SPRY3 has been linked to tumorigenesis, impaired angiogenesis, and developmental disorders. Deletion of SPRY3 in the pseudoautosomal region may contribute to neurodevelopmental phenotypes in certain chromosomal abnormalities. Pathway associations include growth factor receptor signaling, neuronal differentiation, and vascular development. In disease models, SPRY3 acts as a tumor suppressor through attenuation of mitogenic signaling.

The SPRY3 antibody from NSJ Bioreagents is a valuable tool for studying growth factor signaling regulation, angiogenesis, and neurodevelopmental pathways.

UniProt: O43610