CD3D antibody (AA 1-171)

Catalog No. ABIN7868243

This Mouse Monoclonal antibody specifically detects CD3D in IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD3D antibody (AA 1-171) (ABIN7868243)

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3D antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CD3D-8898

Product Details anti-CD3D Antibody

Binding Specificity: AA 1-171

Purpose: CD3 delta Antibody

Purification: Protein A/G affinity

Immunogen: A recombinant fragment (within amino acids 1-171) of human CD3D protein was used as the immunogen for the CD3 delta antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the CD3 delta antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the CD3 delta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for CD3D

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Alternative Name: CD3 delta

Background: The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of g and e chains (ge), a heterodimer of d and e chains (de) and a homodimer of two z chains (zz) or a heterodimer of z and h chains (zh). The z and h chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The g, e and d chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the z chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to the z chain ITAMs has been solved.

UniProt: P04234

Pathways: TCR Signaling, CXCR4-mediated Signaling Events