UBE2F antibody (AA 1-185)

Catalog No. ABIN7868319

The Rabbit Polyclonal anti-UBE2F antibody is suitable to detect UBE2F in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IF, ICC and FACS.

Quick Overview for UBE2F antibody (AA 1-185) (ABIN7868319)

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2F antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-UBE2F Antibody

Binding Specificity: AA 1-185

Purpose: UBE2F Antibody / NEDD8-conjugating enzyme E2 F

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human UBE2F recombinant protein (Position: M1-R185) was used as the immunogen for the UBE2F antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the UBE2F antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the UBE2F antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Alternative Name: UBE2F

Background: UBE2F antibody detects NEDD8-conjugating enzyme E2 F, an E2 ubiquitin-like conjugating enzyme that catalyzes neddylation of cullin proteins, regulating cullin-RING ligase (CRL) activity. The UniProt recommended name is NEDD8-conjugating enzyme E2 F (UBE2F). This enzyme cooperates with the NEDD8-activating enzyme (NAE1/UBA3) and specific E3 ligases to attach NEDD8 to target substrates, modulating protein degradation pathways.

Functionally, UBE2F antibody identifies a 185-amino-acid cytoplasmic enzyme that activates and transfers NEDD8 to cullin 5 and other CRL components. UBE2F works specifically with the E3 ligase RBX2 (RNF7), forming a selective neddylation module distinct from UBE2M (UBC12). Through this modification, UBE2F regulates CRL activity, affecting cell cycle progression, signal transduction, and stress responses.

The UBE2F gene is located on chromosome 5q31.2 and is expressed in multiple tissues, including testis, brain, and lung. Its expression is cell cycle-dependent and induced under oxidative stress. UBE2F ensures timely activation of CRLs, thereby influencing ubiquitin-mediated degradation of regulatory proteins such as p27 and HIF1A.

Pathologically, dysregulation of UBE2F contributes to cancer and oxidative stress-related disorders. Overexpression promotes degradation of tumor suppressors, while inhibition can stabilize proteins that restrain cell proliferation. UBE2F has emerged as a therapeutic target for modulating proteostasis and redox balance in malignant cells.

UBE2F antibody is suitable for western blotting, immunoprecipitation, and immunofluorescence to detect neddylation components and study ubiquitin-like modification systems. NSJ Bioreagents provides validated UBE2F antibody reagents for investigating protein turnover, neddylation, and cellular signaling.

Structurally, UBE2F contains a conserved catalytic cysteine residue within its E2 core domain, forming a thioester bond with activated NEDD8. This structure enables transfer to substrate-bound E3 ligases. This antibody helps characterize UBE2F's mechanistic role in the neddylation cascade and its impact on CRL activity.

UniProt: Q969M7