CD69 antibody (AA 1-199)

Catalog No. ABIN7868359

The Rabbit Polyclonal anti-CD69 antibody (ABIN7868359) specifically detects CD69 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for CD69 antibody (AA 1-199) (ABIN7868359)

Target: CD69

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD69 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CD69 Antibody

Binding Specificity: AA 1-199

Purpose: CD69 Antibody

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human CD69 recombinant protein (Position: M1-K199) was used as the immunogen for the CD69 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CD69 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CD69 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for CD69

Target: CD69

Alternative Name: CD69

Background: CD69 antibody detects Early activation antigen CD69, encoded by the CD69 gene on chromosome 12p13.31. CD69 antibody is widely used in immunology research as CD69 is one of the earliest markers of lymphocyte activation. CD69 is a type II transmembrane C-type lectin receptor expressed on T cells, B cells, NK cells, and other leukocytes shortly after stimulation. It functions in immune regulation, signaling, and tissue retention of lymphocytes, making it a key marker of immune activation and inflammation.

Structurally, CD69 is a ~28-32 kDa disulfide-linked homodimer composed of an extracellular C-type lectin-like domain, a transmembrane region, and a short cytoplasmic tail. The lectin domain mediates protein interactions, while the transmembrane region associates with signaling molecules. CD69 is rapidly upregulated at the cell surface following antigen recognition or cytokine stimulation, making it a sensitive marker of early activation.

Functionally, CD69 regulates immune cell proliferation, cytokine production, and migration. It modulates signaling pathways downstream of the T cell receptor and integrins. Importantly, CD69 binds sphingosine-1-phosphate receptor 1 (S1PR1) and promotes its internalization, reducing lymphocyte egress from lymphoid organs. Through this mechanism, CD69 contributes to tissue retention of immune cells during immune responses. Researchers use CD69 antibody to study lymphocyte activation, tissue homing, and inflammation.

Clinically, CD69 is an important biomarker of immune activation in infection, autoimmune disease, and cancer. Elevated CD69 expression is associated with autoimmune pathologies such as lupus and rheumatoid arthritis. It has also been used to monitor immune responses in HIV infection and cancer immunotherapy. In oncology, tumor-infiltrating lymphocytes expressing CD69 often indicate active immune engagement. NSJ Bioreagents provides CD69 antibody for immunology, oncology, and translational research applications.

Experimentally, CD69 antibody is widely used in flow cytometry to monitor lymphocyte activation, in immunohistochemistry to study tissue infiltration, and in western blotting to confirm expression. Functional assays using CD69 antibody help evaluate lymphocyte responses to antigenic or cytokine stimuli.

UniProt: Q07108