Neurogenin 3 antibody (AA 1-200)

Catalog No. ABIN7868673

The Mouse Monoclonal anti-Neurogenin 3 antibody is suitable to detect Neurogenin 3 in samples from Human. It has been validated for IHC (p).

Quick Overview for Neurogenin 3 antibody (AA 1-200) (ABIN7868673)

Target: Neurogenin 3 (NEUROG3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Neurogenin 3 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NGN3-7698

Product Details anti-Neurogenin 3 Antibody

Binding Specificity: AA 1-200

Purpose: NEUROG3 Antibody / Neurogenin 3 / NGN3

Purification: Protein A/G affinity

Immunogen: A recombinant partial protein (within amino acids 1-200) from the human protein was used as the immunogen for the NEUROG3 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the NEUROG3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the NEUROG3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Neurogenin 3

Target: Neurogenin 3 (NEUROG3)

Alternative Name: NEUROG3

Background: This antigen is a transcription factor that interacts with NKX2-2 to regulation transcription of NEUROD1. The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the neurogenin gene promoter. Neurogenin 3 is involved in the initial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, NeuroD, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of NeuroD in a bHLH cascade. Neurogenin 3 activates the expression of NeuroD at the onset of islet cell differentiation through box sequences E1 and E3 in the NeuroD promoter.

UniProt: Q9Y4Z2