NKX2-1 antibody (AA 1-200)

Catalog No. ABIN7868683

The Mouse Monoclonal anti-NKX2-1 antibody (Clone NX2-1-9031) (ABIN7868683) specifically detects NKX2-1 in IHC (p). The antibody is reactive with Human samples.

Quick Overview for NKX2-1 antibody (AA 1-200) (ABIN7868683)

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NKX2-1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NX2-1-9031

Product Details anti-NKX2-1 Antibody

Binding Specificity: AA 1-200

Purpose: NKX2.1 Antibody / TTF-1

Purification: Protein A/G affinity

Immunogen: A recombinant fragment (within amino acids 1-200) of human TTF-1 protein was used as the immunogen for the NKX2.1 antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the NKX2.1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the NKX2.1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for NKX2-1

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Alternative Name: NKX2.1

Background: Recognizes a protein of 40 kDa, identified as Homeobox protein Nkx-2.1, also called Thyroid transcription factor-1 (TTF-1). NKX2.1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-NKX2.1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. NKX2.1/TTF-1 reactivity is also seen in thyroid malignancies.

UniProt: P43699

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Glucan Metabolic Process, Feeding Behaviour