SPARC antibody (AA 1-200)

Catalog No. ABIN7868748

The Mouse Monoclonal anti-SPARC antibody is suitable to detect SPARC in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for SPARC antibody (AA 1-200) (ABIN7868748)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: OSTN-3761

Product Details anti-SPARC Antibody

Binding Specificity: AA 1-200

Purpose: SPARC Antibody / Secreted protein acidic and rich in cysteine / Osteonectin (azide and preservative free)

Purification: Protein G affinity

Immunogen: A portion of amino acids 1-200 from human SPARC protein was used as the immunogen for the SPARC antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the SPARC antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Aliquot the SPARC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC

Background: SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.

UniProt: P09486

Pathways: Autophagy