Recombinant SPARC antibody (AA 1-200)

Catalog No. ABIN7868751

The Rabbit Monoclonal anti-SPARC antibody is suitable to detect SPARC in samples from Human. It has been validated for IHC (p).

Quick Overview for Recombinant SPARC antibody (AA 1-200) (ABIN7868751)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: OSTN-8528R

Product Details anti-SPARC Antibody

Binding Specificity: AA 1-200

Purpose: SPARC Antibody / Osteonectin

Purification: Protein A/G affinity

Immunogen: A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the SPARC antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the SPARC antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SPARC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC

Background: SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.

UniProt: P09486

Pathways: Autophagy