T-Bet antibody (AA 1-200)

Catalog No. ABIN7868765

This Mouse Monoclonal antibody specifically detects T-Bet in IHC (p). It exhibits reactivity toward Human.

Quick Overview for T-Bet antibody (AA 1-200) (ABIN7868765)

Target: T-Bet

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This T-Bet antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TBX21-6724

Product Details anti-T-Bet Antibody

Binding Specificity: AA 1-200

Purpose: TBX21 Antibody / T-bet

Purification: Protein A/G affinity

Immunogen: A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the TBX21 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the TBX21 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the TBX21 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for T-Bet

Target: T-Bet

Alternative Name: TBX21

Background: T helper (Th) lymphocytes differentiate into two unique subsets, Th1 and Th2, which differ both in function and in the cytokines they secrete. Th1 and Th2 cytokines promote the growth and differentiation of their subset, and inhibit the growth and differentiation of the opposing subset. T-bet (T-box expressed in T cells) is a Th1-specific T-box transcription factor that controls the expression of the Th1 cytokine, IFN-g. T-bet also converts effector Th2 cells into the opposing Th1 subset. T-bet is selectively expressed in Th1 cells. The level of T-bet expression is increased by signals mediated by the T cell receptor (TCR). IL-12 also induces an increase in the level of T-bet. T-bet was originally isolated from nuclear extracts of resting and PMA/ionomycin-activated AE7 cells. It is expressed in low levels in AE7 cells, and in increased levels in stimulated AE7.

UniProt: Q9UL17