GINS3 antibody (AA 1-216)

Catalog No. ABIN7868834

This Rabbit Polyclonal antibody specifically detects GINS3 in WB, ELISA, ICC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for GINS3 antibody (AA 1-216) (ABIN7868834)

Target: GINS3 (GINS Complex Subunit 3 (Psf3 Homolog) (GINS3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GINS3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-GINS3 Antibody

Binding Specificity: AA 1-216

Purpose: GINS3 Antibody / GINS protein subunit 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human GINS3 recombinant protein (Position: M1-D216) was used as the immunogen for the GINS3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GINS3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GINS3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GINS3

Target: GINS3 (GINS Complex Subunit 3 (Psf3 Homolog) (GINS3))

Alternative Name: GINS3

Background: GINS3 antibody detects DNA replication complex GINS protein subunit 3, a component of the GINS complex essential for the initiation and elongation phases of eukaryotic DNA replication. The UniProt recommended name is DNA replication complex GINS protein subunit 3 (GINS3). This protein is a critical element of the CMG helicase complex, which unwinds DNA strands during replication fork progression.

Functionally, GINS3 antibody identifies a 223-amino-acid protein that forms part of the heterotetrameric GINS complex along with GINS1 (Sld5), GINS2 (Psf2), and GINS4 (Psf3). The GINS complex interacts with Cdc45 and the MCM2-7 helicase to form the CMG (Cdc45-MCM-GINS) complex, which drives replication fork unwinding and DNA synthesis. GINS3 stabilizes this complex, ensuring efficient replication initiation and fork progression during S phase.

The GINS3 gene is located on chromosome 16q24.1 and encodes a conserved protein expressed in proliferating cells and tissues undergoing rapid growth. GINS3 functions as a scaffold subunit, mediating interactions between GINS partners and replication factors such as DNA polymerase epsilon. Its depletion leads to stalled replication forks, DNA damage accumulation, and activation of checkpoint signaling, highlighting its essential role in genome duplication.

In cell cycle regulation, GINS3 contributes to origin firing and replication timing control. It is recruited to replication origins during early S phase and remains associated with active replication forks. Dysregulation of GINS3 or other CMG components disrupts DNA replication dynamics and can result in replication stress, chromosomal instability, and tumorigenesis. Overexpression of GINS subunits has been observed in cancers characterized by high proliferation rates, including breast, ovarian, and colorectal cancers.

GINS3 antibody is widely used in cell cycle, DNA replication, and cancer research. It is suitable for immunoblotting, immunofluorescence, and chromatin fractionation assays to examine GINS3 expression and complex formation. This antibody supports studies of replication fork mechanics, checkpoint signaling, and DNA damage responses. In oncology, it helps assess replication stress pathways and therapeutic sensitivity to DNA synthesis inhibitors.

Structurally, GINS3 adopts a beta-sheet-rich fold that interfaces with GINS1 and GINS2 to stabilize the quaternary complex. It acts as a bridge linking DNA helicase and polymerase activities at replication forks. NSJ Bioreagents provides GINS3 antibody reagents validated for use in DNA replication, cell cycle progression, and genomic integrity research.

UniProt: Q9BRX5