FAM60A antibody (AA 1-221)

Catalog No. ABIN7868850

The Rabbit Polyclonal anti-FAM60A antibody is suitable to detect FAM60A in samples from Human and Rat. It has been validated for ELISA, WB, IF, FACS and IHC (p).

Quick Overview for FAM60A antibody (AA 1-221) (ABIN7868850)

Target: FAM60A (Family with Sequence Similarity 60, Member A (FAM60A))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAM60A antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FAM60A Antibody

Binding Specificity: AA 1-221

Purpose: FAM60A Antibody / SINHCAF

Purification: Antigen affinity purified

Immunogen: E. coli-derived recombinant human protein (amino acids M1-W221) was used as the immunogen for the FAM60A antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the FAM60A antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the FAM60A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for FAM60A

Target: FAM60A (Family with Sequence Similarity 60, Member A (FAM60A))

Alternative Name: FAM60A

Background: FAM60A, also known as Protein FAM60A, Tera protein homolog and SIN3-HDAC complex-associated factor (SINHCAF), is a subunit of the Sin3 deacetylase complex (Sin3/HDAC) that is important for the repression of genes. The SIN3A-HDAC complex deacetylates histones thereby repressing gene transcription. FAM60A specifically impacts genes encoding components of the TGF-beta signaling pathway and promoters of important proteins like cyclin D1. FAM60A activity peaks during G1 and S phases of the cell cycle and is up regulated in many carcinoma and tumor tissues. Additionally, loss of FAM60A leads to a change in cell morphology, an increase in cell migration, increased histone acetylation at the cyclin D1 promoter and elevated levels of cyclin D1 mRNA and protein. Furthermore, depletion of FAM60A altered the periodic association of HDAC1 with the cyclin D1 promoter, increased cyclin D1 expression at all cell cycle phases, and caused premature S phase entry.

UniProt: Q9NP50