GINS4 antibody (AA 1-223)

Catalog No. ABIN7868852

This Rabbit Polyclonal antibody specifically detects GINS4 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GINS4 antibody (AA 1-223) (ABIN7868852)

Target: GINS4 (GINS Complex Subunit 4 (Sld5 Homolog) (GINS4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GINS4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-GINS4 Antibody

Binding Specificity: AA 1-223

Purpose: GINS4 Antibody / GINS complex subunit 4

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SLD5/GINS4 recombinant protein (Position: M1-I223) was used as the immunogen for the GINS4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GINS4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GINS4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GINS4

Target: GINS4 (GINS Complex Subunit 4 (Sld5 Homolog) (GINS4))

Alternative Name: GINS4

Background: GINS4 antibody detects GINS complex subunit 4, encoded by the GINS4 gene. GINS complex subunit 4, also known as SLD5, is one of four essential components of the GINS complex, which plays a central role in DNA replication initiation and elongation. GINS4 antibody provides researchers with a specific reagent for studying DNA replication, genome stability, and cell cycle control.

The GINS complex, composed of GINS1, GINS2, GINS3, and GINS4, forms a stable heterotetramer that interacts with CDC45 and the MCM helicase complex to establish the CMG helicase. Research using GINS4 antibody has demonstrated that the CMG helicase is the core replicative helicase in eukaryotic cells, responsible for unwinding DNA during replication. Loss of GINS4 impairs DNA replication fork progression and genome stability, highlighting its indispensable role.

Studies with GINS4 antibody have revealed that GINS4 interacts with multiple replication factors, including MCM proteins, replication protein A, and DNA polymerases. These interactions coordinate helicase activity with DNA synthesis, ensuring high-fidelity replication. Structural studies further confirm that GINS4 stabilizes the complex and supports helicase activity.

Dysregulation of GINS4 is linked to cancer and genomic instability. Research using GINS4 antibody has shown that elevated GINS4 expression is associated with proliferation and poor prognosis in several cancers, including lung and colorectal cancers. By promoting replication efficiency, cancer cells exploit GINS4 to sustain high growth rates. Conversely, depletion of GINS4 induces replication stress and apoptosis, underscoring its therapeutic potential as a replication factor target.

GINS4 antibody is widely applied in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting quantifies protein levels, immunohistochemistry identifies proliferative zones with high replication activity, and immunoprecipitation demonstrates interaction with replication machinery. These approaches make GINS4 antibody invaluable in DNA replication research.

By supplying validated GINS4 antibody reagents, NSJ Bioreagents supports studies into DNA replication, cell cycle regulation, and cancer biology. Detection of GINS complex subunit 4 provides insight into how replication machinery safeguards genome stability.

UniProt: Q9BRT9

Pathways: DNA Replication, Synthesis of DNA