HDHD1 antibody (AA 1-228)

Catalog No. ABIN7868873

This Rabbit Polyclonal antibody specifically detects HDHD1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for HDHD1 antibody (AA 1-228) (ABIN7868873)

Target: HDHD1 (Haloacid Dehalogenase-Like Hydrolase Domain Containing 1 (HDHD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDHD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HDHD1 Antibody

Binding Specificity: AA 1-228

Purpose: PUDP Antibody / Pseudouridine 5-phosphatase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PUDP recombinant protein (Position: M1-E228) was used as the immunogen for the PUDP antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PUDP antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PUDP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for HDHD1

Target: HDHD1 (Haloacid Dehalogenase-Like Hydrolase Domain Containing 1 (HDHD1))

Alternative Name: PUDP

Background: PUDP antibody detects Pseudouridine 5'-phosphatase, an enzyme involved in nucleotide metabolism and RNA modification recycling. Encoded by the PUDP gene on chromosome 10q23.31, this enzyme catalyzes the dephosphorylation of pseudouridine 5'-phosphate, a modified nucleoside derived from RNA turnover. By recycling pseudouridine into usable intermediates, PUDP contributes to nucleotide homeostasis and RNA degradation pathways essential for cellular metabolism.

PUDP is a member of the haloacid dehalogenase (HAD) superfamily of hydrolases and shares structural features with sugar phosphatases, including a conserved catalytic Asp residue that facilitates phosphate hydrolysis. The enzyme functions primarily in the cytoplasm and exhibits substrate specificity for pseudouridine monophosphate over canonical nucleotides. PUDP activity supports RNA salvage by clearing modified nucleosides generated from rRNA and tRNA decay.

The PUDP antibody is used in enzymology, RNA metabolism, and nucleotide biochemistry research to study RNA turnover and modified nucleotide processing. Western blot analysis identifies a 31 kilodalton band corresponding to PUDP, while immunofluorescence shows diffuse cytoplasmic localization consistent with its metabolic role. This antibody provides a useful reagent for monitoring nucleotide recycling and evaluating enzymatic regulation in metabolic and stress-response pathways.

Altered expression of PUDP has been associated with cancer, where metabolic reprogramming changes nucleotide flux and RNA stability. By participating in modified nucleotide clearance, PUDP helps maintain nucleotide pool balance and prevents accumulation of potentially toxic intermediates. The PUDP antibody supports studies into RNA degradation, nucleotide recycling, and metabolic adaptation. NSJ Bioreagents validates this antibody for its applications ensuring specificity and reproducibility for nucleotide metabolism research.

UniProt: Q08623