TGIF2 antibody (AA 1-237)

Catalog No. ABIN7868891

This Rabbit Polyclonal antibody specifically detects TGIF2 in ELISA, WB, IF, ICC and IP. It exhibits reactivity toward Human.

Quick Overview for TGIF2 antibody (AA 1-237) (ABIN7868891)

Target: TGIF2 (TGFB-Induced Factor Homeobox 2 (TGIF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TGIF2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-TGIF2 Antibody

Binding Specificity: AA 1-237

Purpose: TGIF2 Antibody / TG-interacting factor 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TGIF2 recombinant protein (Position: M1-Q237) was used as the immunogen for the TGIF2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TGIF2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TGIF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TGIF2

Target: TGIF2 (TGFB-Induced Factor Homeobox 2 (TGIF2))

Alternative Name: TGIF2

Background: TGIF2 antibody detects TG-interacting factor 2, a homeodomain transcriptional repressor that modulates TGF-beta signaling and developmental gene expression. The UniProt recommended name is TG-interacting factor 2 (TGIF2). This nuclear protein belongs to the TALE (three-amino acid loop extension) class of homeobox proteins and functions as a DNA-binding corepressor that inhibits Smad-mediated transcription.

Functionally, TGIF2 antibody identifies a 237-amino-acid protein localized in the nucleus, where it binds DNA at consensus homeobox sites and interacts with transcriptional repressors such as CtBP and HDACs. TGIF2 represses TGF-beta target genes by blocking Smad binding to promoters, fine-tuning cellular differentiation, proliferation, and morphogenesis during embryonic development.

The TGIF2 gene is located on chromosome 20q11.2 and is expressed in embryonic tissues and adult organs including brain, kidney, and testis. TGIF2 acts as a developmental regulator controlling anterior-posterior patterning and organ formation through transcriptional repression mechanisms.

Pathologically, TGIF2 overexpression has been observed in several cancers, including ovarian and gastric tumors, where it promotes cell proliferation and epithelial-mesenchymal transition. Gene duplications or deletions involving TGIF2 have been associated with holoprosencephaly and craniofacial malformations. Research using TGIF2 antibody supports studies in transcriptional repression, developmental biology, and oncogenesis.

TGIF2 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect homeodomain transcription factors. NSJ Bioreagents provides TGIF2 antibody reagents optimized for developmental and transcriptional research applications.

Structurally, TG-interacting factor 2 contains a homeobox DNA-binding domain and a CtBP-binding motif that mediate its repressor activity. This antibody aids in analyzing TGIF2's role in gene regulation, morphogenesis, and signaling cross-talk.

UniProt: Q9GZN2