NOTO antibody (AA 1-241)

Catalog No. ABIN7868905

The Rabbit Polyclonal anti-NOTO antibody (ABIN7868905) specifically detects NOTO in WB. The antibody is reactive with Zebrafish (Danio rerio) samples.

Quick Overview for NOTO antibody (AA 1-241) (ABIN7868905)

Target: NOTO (Notochord Homeobox (NOTO))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOTO antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NOTO Antibody

Binding Specificity: AA 1-241

Purpose: Zebrafish Noto Antibody / Notochord homeobox protein / Floating head

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Noto recombinant protein (amino acids M1-D241) was used as the immunogen for the Zebrafish Noto antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Noto antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Noto antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NOTO

Target: NOTO (Notochord Homeobox (NOTO))

Alternative Name: Noto

Background: Noto, or notochord homeobox protein, is a transcription factor that plays a fundamental role in the formation and patterning of the notochord and tailbud during early embryonic development. In zebrafish, Noto is essential for proper axis formation, neural tube development, and left right asymmetry.

Zebrafish Noto is considered the functional ortholog of the human NOTO gene. Both zebrafish and human versions of this gene encode homeodomain containing transcription factors that are critical for notochord morphogenesis and cilia driven fluid flow, which influences organ laterality during development. Despite some sequence divergence between species, the developmental roles of zebrafish Noto and human NOTO are highly conserved.

In the zebrafish embryo, Noto is expressed in the dorsal organizer, the notochordal plate, and the developing tailbud. It regulates genes involved in axial mesoderm formation and ciliogenesis, and it interacts with other developmental signals including components of the nodal and hedgehog pathways. Loss of Noto function leads to defects in notochord structure, tail elongation, and laterality, including reversed heart and gut looping.

Because of its critical role in vertebrate axis and notochord formation, zebrafish Noto is widely used in developmental biology to study early mesodermal differentiation, organ positioning, and congenital disorders related to axis specification. Its functional conservation with the human NOTO gene also makes it a useful model for investigating the genetic basis of developmental syndromes affecting the spine and body plan.

UniProt: Q90461