BCAP31 antibody (AA 1-242)

Catalog No. ABIN7868908

The Rabbit Polyclonal anti-BCAP31 antibody (ABIN7868908) specifically detects BCAP31 in WB, ELISA, IHC, IF, IP, ICC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for BCAP31 antibody (AA 1-242) (ABIN7868908)

Target: BCAP31 (B-Cell Receptor-Associated Protein 31 (BCAP31))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCAP31 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-BCAP31 Antibody

Binding Specificity: AA 1-242

Purpose: BCAP31 Antibody / B-cell receptor-associated protein 31

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human BAP31/BCAP31 recombinant protein (Position: M1-D242) was used as the immunogen for the BCAP31 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the BCAP31 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the BCAP31 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for BCAP31

Target: BCAP31 (B-Cell Receptor-Associated Protein 31 (BCAP31))

Alternative Name: BCAP31

Background: BCAP31 antibody detects B-cell receptor-associated protein 31, an integral endoplasmic reticulum (ER) membrane protein that functions as a chaperone and adaptor in protein trafficking and apoptosis regulation. BCAP31 is a member of the BCAP31 family, which includes BCAP29, and plays crucial roles in protein quality control, ER-Golgi transport, and caspase-mediated cell death. The BCAP31 antibody is used in cell biology and immunology research to study membrane trafficking, ER stress, and apoptotic signaling.

BCAP31 is encoded by the BCAP31 gene located on the X chromosome (Xp22.13). The protein is approximately 28 kilodaltons and contains three transmembrane domains with a cytoplasmic C-terminal tail that interacts with diverse partners, including BCL2 and procaspase-8. These interactions position BCAP31 at the intersection of the secretory and apoptotic pathways, integrating ER homeostasis with cell death regulation.

The BCAP31 antibody detects a 28 kilodalton band on western blot and shows reticular ER staining consistent with its localization. BCAP31 forms complexes with chaperones such as calnexin and Sec61, assisting in the folding and export of glycoproteins from the ER to the Golgi apparatus. It also mediates ER retention or degradation of misfolded proteins, linking it to the unfolded protein response (UPR).

In apoptosis, BCAP31 acts as a substrate for caspase cleavage, generating pro-apoptotic fragments that amplify death signaling through mitochondria. This dual role allows BCAP31 to serve as both a quality control checkpoint and a stress sensor. Mutations or deletions of BCAP31 cause X-linked intellectual disability syndromes characterized by developmental delay and hearing loss, emphasizing its importance in neuronal maintenance.

BCAP31 also contributes to immune cell signaling and antigen presentation by modulating trafficking of MHC class I molecules. NSJ Bioreagents provides a validated BCAP31 antibody optimized for western blot, immunofluorescence, and ER-Golgi transport analysis, supporting research into protein folding, apoptosis regulation, and membrane trafficking.

UniProt: P51572

Pathways: Positive Regulation of Endopeptidase Activity