CD33 antibody (AA 1-250)

Catalog No. ABIN7868933

This Mouse Monoclonal antibody specifically detects CD33 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD33 antibody (AA 1-250) (ABIN7868933)

Target: CD33

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD33 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: SIGLEC3-3597

Product Details anti-CD33 Antibody

Binding Specificity: AA 1-250

Purpose: SIGLEC3 Antibody / CD33 (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: A recombinant partial protein sequence (within amino acids 1-250) from the human protein was used as the immunogen for the SIGLEC3 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the SIGLEC3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Aliquot the SIGLEC3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for CD33

Target: CD33

Alternative Name: SIGLEC3

Background: Recognizes a 67 kDa glycoprotein, which is identified as CD33. It is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

UniProt: P20138