PHYHD1 antibody (AA 1-250)

Catalog No. ABIN7868947

The Rabbit Polyclonal anti-PHYHD1 antibody (ABIN7868947) specifically detects PHYHD1 in WB and ELISA. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for PHYHD1 antibody (AA 1-250) (ABIN7868947)

Target: PHYHD1 (Phytanoyl-CoA Dioxygenase Domain Containing 1 (PHYHD1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHYHD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PHYHD1 Antibody

Binding Specificity: AA 1-250

Purpose: PHYHD1 Antibody / Phytanoyl-CoA dioxygenase domain-containing protein 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PHYHD1 recombinant protein (Position: M1-Q250) was used as the immunogen for the PHYHD1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PHYHD1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PHYHD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PHYHD1

Target: PHYHD1 (Phytanoyl-CoA Dioxygenase Domain Containing 1 (PHYHD1))

Alternative Name: PHYHD1

Background: PHYHD1 antibody detects Phytanoyl-CoA dioxygenase domain-containing protein 1, a putative oxidative enzyme that may play roles in lipid metabolism and cellular stress responses. The UniProt recommended name is Phytanoyl-CoA dioxygenase domain-containing protein 1 (PHYHD1). This non-heme iron-dependent enzyme is thought to participate in hydroxylation or oxidative reactions linked to fatty acid metabolism and peroxisomal function.

Functionally, PHYHD1 antibody identifies a 290-amino-acid protein localized mainly in the cytoplasm and potentially associated with peroxisomes. PHYHD1 contains a conserved 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase domain that catalyzes oxidation reactions using molecular oxygen and 2-oxoglutarate as cofactors. Although its precise substrates remain under investigation, PHYHD1 may regulate lipid processing, hypoxia response, and reactive oxygen species signaling.

The PHYHD1 gene is located on chromosome 9q34.11 and is expressed in brain, liver, and kidney. Transcription of PHYHD1 is induced by cellular stress and metabolic cues, suggesting it plays a role in adaptive metabolic regulation. Comparative genomics studies indicate conservation across vertebrates, implying fundamental metabolic importance.

Pathologically, alterations in PHYHD1 expression have been associated with gliomas and metabolic disorders involving peroxisomal dysfunction. Reduced PHYHD1 levels may impair fatty acid oxidation and redox homeostasis, while overexpression correlates with enhanced metabolic flexibility in cancer cells. Research using PHYHD1 antibody supports studies in lipid oxidation, metabolic adaptation, and enzymatic regulation under hypoxia.

PHYHD1 antibody is validated for western blotting, immunofluorescence, and ELISA to detect 2OG-dependent oxygenases. NSJ Bioreagents provides PHYHD1 antibody reagents optimized for research in oxidative metabolism, enzymology, and metabolic disease mechanisms.

Structurally, Phytanoyl-CoA dioxygenase domain-containing protein 1 features a double-stranded beta-helix fold typical of 2OG oxygenases, with a conserved His-Asp-His metal-binding triad that coordinates Fe(II). This structure enables catalytic hydroxylation reactions essential for lipid and oxygen metabolism. This antibody facilitates analysis of PHYHD1's role in oxidative biochemistry, peroxisomal function, and metabolic stress response.

UniProt: Q5SRE7