IMPA1 antibody (AA 1-277)

Catalog No. ABIN7869035

This Rabbit Polyclonal antibody specifically detects IMPA1 in WB, ELISA, FACS and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IMPA1 antibody (AA 1-277) (ABIN7869035)

Target: IMPA1 (Inositol(myo)-1(or 4)-Monophosphatase 1 (IMPA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IMPA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-IMPA1 Antibody

Binding Specificity: AA 1-277

Purpose: IMPA1 Antibody / Inositol monophosphatase 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human IMPA1 recombinant protein (Position: M1-D277) was used as the immunogen for the IMPA1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the IMPA1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the IMPA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for IMPA1

Target: IMPA1 (Inositol(myo)-1(or 4)-Monophosphatase 1 (IMPA1))

Alternative Name: IMPA1

Background: IMPA1 antibody recognizes Inositol monophosphatase 1, a magnesium-dependent enzyme that catalyzes the dephosphorylation of inositol monophosphate to free inositol, a crucial step in the phosphatidylinositol signaling pathway. The IMPA1 gene encodes a cytosolic enzyme essential for recycling inositol phosphates derived from the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2). This reaction is critical for maintaining inositol supply for the synthesis of phosphoinositides, which regulate intracellular signaling, neurotransmission, and osmoregulation.

IMPA1 is expressed abundantly in brain, kidney, and liver, reflecting its central role in inositol metabolism. In neurons, IMPA1 supports the phosphoinositide cycle, enabling sustained neurotransmitter signaling via inositol trisphosphate and diacylglycerol pathways. Pharmacologically, IMPA1 is a well-known molecular target of lithium, a mood-stabilizing drug used in the treatment of bipolar disorder. Lithium inhibits IMPA1 activity, leading to inositol depletion and modulation of neuronal signaling, supporting the i 1/2inositol depletion hypothesisi 1/2 for mood stabilization.

The human IMPA1 gene is located on chromosome 8q21.13 and encodes a 277-amino acid protein that functions as a homodimer. The enzyme binds magnesium ions within its active site to hydrolyze phosphate from a range of inositol monophosphate substrates. Structural studies have revealed conserved residues critical for catalysis and lithium binding. Genetic or functional alterations in IMPA1 have been associated with neurological disorders, cognitive deficits, and certain metabolic diseases. Experimental evidence also implicates IMPA1 in maintaining cellular osmotic balance, particularly in renal epithelial cells where inositol acts as a compatible osmolyte.

Immunohistochemical analysis using IMPA1 antibody shows strong cytoplasmic staining in neurons, glial cells, renal tubules, and hepatocytes. The antibody is useful for studying inositol phosphate metabolism, lithium pharmacology, and neuronal signaling pathways. IMPA1 antibody from NSJ Bioreagents supports research into inositol-dependent biochemical networks and the molecular mechanisms underlying psychiatric and neurological disease.

UniProt: P29218