Syntaxin 4 antibody (AA 1-278)

Catalog No. ABIN7869039

The Rabbit Polyclonal anti-Syntaxin 4 antibody is suitable to detect Syntaxin 4 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IP, IF, IHC, ICC and FACS.

Quick Overview for Syntaxin 4 antibody (AA 1-278) (ABIN7869039)

Target: Syntaxin 4 (STX4)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syntaxin 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Syntaxin 4 Antibody

Binding Specificity: AA 1-278

Purpose: STX4 Antibody / Syntaxin 4

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Syntaxin 4/STX4 recombinant protein (Position: M1-I278) was used as the immunogen for the STX4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the STX4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the STX4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Syntaxin 4

Target: Syntaxin 4 (STX4)

Alternative Name: STX4

Background: STX4 antibody detects Syntaxin 4, a membrane-anchored SNARE protein that mediates vesicle fusion and exocytosis at the plasma membrane. Encoded by the STX4 gene on chromosome 16p11.2, this t-SNARE protein participates in vesicular trafficking pathways, particularly in insulin-stimulated glucose transporter translocation, neurotransmitter release, and immune cell secretion. Syntaxin 4 plays a central role in membrane docking and fusion by forming SNARE complexes with SNAP23 and VAMP2 or VAMP3.

Structurally, Syntaxin 4 is a 273-amino-acid membrane protein of approximately 33 kilodaltons containing a C-terminal transmembrane anchor, a SNARE motif required for complex formation, and an N-terminal regulatory domain. It localizes to the plasma membrane and cytoplasmic vesicles in diverse tissues including adipose, neuronal, and epithelial cells. Upon stimulation, Syntaxin 4 assembles into heterotrimeric SNARE complexes that catalyze the fusion of transport vesicles with target membranes.

The STX4 antibody is widely used in cell biology, metabolism, and neurophysiology research to study vesicular trafficking, membrane fusion, and regulated exocytosis. Western blot analysis detects a 33 kilodalton band corresponding to Syntaxin 4, while immunofluorescence reveals peripheral and punctate membrane staining. This antibody provides a reliable reagent for analyzing vesicle dynamics and fusion machinery in secretory systems.

Functionally, Syntaxin 4 is essential for insulin-dependent GLUT4 trafficking in adipocytes and muscle cells, linking it to glucose uptake and metabolic homeostasis. It also contributes to immune cell secretion of cytokines and cytotoxic granules, as well as neurotransmitter release in the nervous system. Disruption of STX4 impairs exocytic processes and contributes to metabolic disorders such as type 2 diabetes. The STX4 antibody supports studies exploring SNARE-mediated exocytosis, vesicle targeting, and signal-dependent membrane fusion. NSJ Bioreagents validates this antibody for its applications, ensuring accuracy and reproducibility in vesicular transport studies.

UniProt: Q12846

Pathways: Synaptic Vesicle Exocytosis