POLR1E antibody (AA 1-291)

Catalog No. ABIN7869059

This Rabbit Polyclonal antibody specifically detects POLR1E in WB, ELISA, IP, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for POLR1E antibody (AA 1-291) (ABIN7869059)

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1E antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-POLR1E Antibody

Binding Specificity: AA 1-291

Purpose: POLR1E Antibody / DNA-directed RNA polymerase I subunit E / PAF53

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human POLR1E recombinant protein (Position: M1-Q291) was used as the immunogen for the POLR1E antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the POLR1E antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the POLR1E antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for POLR1E

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Alternative Name: POLR1E

Background: POLR1E antibody detects DNA-directed RNA polymerase I subunit E (also known as PAF53), a core component of the RNA polymerase I complex responsible for synthesizing ribosomal RNA (rRNA) precursors. Encoded by the POLR1E gene on chromosome 9q34.11, this protein is essential for rRNA transcription and ribosome biogenesis. POLR1E interacts with other polymerase I subunits, transcription initiation factors, and nucleolar scaffolding proteins to regulate transcription of the 47S rRNA gene cluster. By supporting nucleolar organization and transcriptional efficiency, it plays a fundamental role in cell growth and proliferation.

POLR1E localizes to the nucleolus and associates with the transcription initiation factor SL1 and upstream binding factor (UBF) to form a preinitiation complex at rDNA promoters. Its C-terminal domain mediates binding to RNA polymerase I subunit RPA1 and other core enzyme components, facilitating enzyme assembly and promoter clearance. Loss of POLR1E disrupts nucleolar architecture and reduces rRNA synthesis, leading to impaired ribosome production and cell-cycle arrest. This makes it a critical determinant of nucleolar integrity and growth control.

The POLR1E antibody is widely used in molecular biology, cancer research, and nucleolar studies to assess RNA polymerase I activity and rRNA transcription regulation. Western blot analysis identifies a 53 kilodalton band corresponding to POLR1E, while immunofluorescence reveals punctate nucleolar staining. Because RNA polymerase I transcription drives ribosome production, alterations in POLR1E expression are linked to tumor growth and metabolic adaptation. Increased expression has been observed in rapidly dividing cells, whereas downregulation occurs under cellular stress or growth inhibition.

In addition to its role in transcription, POLR1E contributes to the nucleolar stress response by coordinating signaling between rRNA synthesis and p53 activation. Defects in polymerase I assembly or activity trigger nucleolar stress pathways that stabilize p53, resulting in growth arrest. The POLR1E antibody enables exploration of these mechanisms and supports investigations into ribosomopathies, tumorigenesis, and cellular aging. NSJ Bioreagents provides this antibody validated for its applications, ensuring precise and reliable detection across model systems.

UniProt: Q9GZS1