USP14 antibody (AA 1-30)

Catalog No. ABIN7869109

The Rabbit Polyclonal anti-USP14 antibody (ABIN7869109) specifically detects USP14 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for USP14 antibody (AA 1-30) (ABIN7869109)

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-USP14 Antibody

Binding Specificity: AA 1-30

Purpose: USP14 Antibody

Purification: SAS precipitation

Immunogen: A portion of amino acids 1-30 from the human protein was used as the immunogen for the USP14 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the USP14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the USP14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for USP14

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Alternative Name: USP14

Background: Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

UniProt: P54578