CAMKK2 antibody (AA 1-349)

Catalog No. ABIN7869230

This Rabbit Polyclonal antibody specifically detects CAMKK2 in WB, ELISA, IHC, FACS, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CAMKK2 antibody (AA 1-349) (ABIN7869230)

Target: CAMKK2 (Calcium/calmodulin-Dependent Protein Kinase Kinase 2, beta (CAMKK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMKK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CAMKK2 Antibody

Binding Specificity: AA 1-349

Purpose: CAMKK2 Antibody / Calcium/calmodulin-dependent protein kinase kinase 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human CAMKK2 recombinant protein (Position: M1-G349) was used as the immunogen for the CAMKK2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CAMKK2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CAMKK2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for CAMKK2

Target: CAMKK2 (Calcium/calmodulin-Dependent Protein Kinase Kinase 2, beta (CAMKK2))

Alternative Name: CAMKK2

Background: CAMKK2 antibody detects Calcium/calmodulin-dependent protein kinase kinase 2, encoded by the CAMKK2 gene on chromosome 12q24.31. CAMKK2 antibody is widely used to investigate this serine/threonine kinase, which phosphorylates and activates downstream kinases including CAMK1, CAMK4, and AMPK. By integrating calcium/calmodulin signals, CAMKK2 regulates diverse processes such as neuronal plasticity, energy metabolism, and inflammation. CAMKK2 is expressed in brain, liver, adipose tissue, and immune cells, highlighting its broad physiological roles.

Structurally, CAMKK2 contains an N-terminal catalytic domain, a regulatory segment with autoinhibitory and calmodulin-binding motifs, and a C-terminal extension that influences subcellular localization. Calcium/calmodulin binding relieves autoinhibition and activates the kinase. CAMKK2 also undergoes phosphorylation by upstream kinases and autophosphorylation, further modulating activity. Its ability to phosphorylate AMPK connects calcium signaling to metabolic regulation, while CAMK1/4 activation links CAMKK2 to transcriptional control in neurons.

Functionally, CAMKK2 contributes to long-term potentiation, memory formation, and neuronal differentiation. It modulates synaptic activity through regulation of gene expression programs. In metabolism, CAMKK2 activates AMPK in response to calcium flux, influencing glucose uptake, fatty acid oxidation, and energy homeostasis. In immune cells, CAMKK2 promotes pro-inflammatory gene expression, linking it to inflammatory and metabolic diseases. Dysregulation of CAMKK2 activity has been associated with obesity, diabetes, cardiovascular disease, and cancer. Pharmacological inhibitors of CAMKK2 are being investigated for therapeutic applications in metabolic disorders and prostate cancer.

Clinically, CAMKK2 overexpression is reported in prostate cancer, where it promotes androgen receptor signaling and tumor progression. Loss of CAMKK2 activity impairs tumor growth, highlighting its therapeutic potential. Neurologically, CAMKK2 dysfunction may contribute to psychiatric conditions including anxiety, depression, and schizophrenia, where altered calcium signaling disrupts synaptic plasticity. Researchers use CAMKK2 antibody to assess expression and activity across these contexts. Applications include western blotting to detect isoforms, immunohistochemistry for tissue distribution, and immunoprecipitation for substrate identification. NSJ Bioreagents provides CAMKK2 antibody as a high-quality reagent for research in neuroscience, metabolism, and oncology.

UniProt: Q96RR4

Pathways: AMPK Signaling